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KMID : 0378019950380010011
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New Medical Journal
1995 Volume.38 No. 1 p.11 ~ p.20
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전종희
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Abstract
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Background : Disinfection is fundamental to the intelligent practice of medicine. There are many techniques and chemicals for disinfection and sterilization. But these conventional methods are expensive and have some limitations such as showing allergic reaction or causing environmental pollution. Electrolyzed-oxidizing water, which is produced by electrolysis of simple tap water and small amount of pure sodium chloride, were reported to have bactericidal activity against methicillin resistance Staphylococcus aureus and Pseudomonas aeruginosa etc. And this is not costly and does not cause any clinical problems and environmental pollution.
Methods : Bacteria and yeast were exposed to electrolyzed-oxidizing water taken from Super Oxseed a 10001 machine. Pure culture of methicillin-susceptible S. aureus, methicillin- resistance S. aureus, Escherichia coli, P. aeruginosa, Candida albicans, and spore forming Bacillus spp. were used. Survivals at 0.5, 1, 2, 4, 8, 16, and 32 minutes were measured by plating the 0.01 mL of bacteria and oxidizing water mixture and counting the colonies.
Results : Electrolyzed-oxidizing water killed all of 5 strains of methicillin-susceptible S. aureus and 5 strains of methicillin-resistant S. aureus within 30 seconds. All of 10 strains of E. coli, 10 strains of P. aeruginosa and 5 strains of C. albicans were also killed within 30 seconds. But spore-forming Bacillus spp. were not killed after4 minutes except ATCC 19637 and 11778 strain.
Conclusions : This study showed that oxidizing water was effective for the disinfection of commonly occurring bacteria and some yeast, but a longer exposure time was necessary in case of spore-forming gram-positive bacteria.
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KEYWORD
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